![]() ![]() ![]() Recent advances in high-pressure freezing: equipment- and specimen-loading methods. in Protein Localization by Fluorescence Microscopy: A Practical Approach (ed. Aberrations in confocal fluorescence microscopy induced by mismatches in refractive index. Seeing is believing? A beginners’ guide to practical pitfalls in image acquisition. The 39 steps: a cautionary tale of quantitative 3-D fluorescence microscopy. A visual summary of this tutorial is available as a poster ( ). Finally, guidelines for analyzing and presenting confocal images in a way that maintains the quantitative nature of the data are presented, and statistical analysis is discussed. Common pitfalls such as photobleaching and cross-talk are addressed, as well as several troubling instrumentation problems that may prevent the acquisition of quantitative data. Suggestions are offered for planning unbiased and rigorous confocal microscope experiments. In this tutorial, the researcher is guided through all aspects of acquiring quantitative confocal microscopy images, including optimizing sample preparation for fixed and live cells, choosing the most suitable microscope for a given application and configuring the microscope parameters. However, generating meaningful data using confocal microscopy requires careful planning and a thorough understanding of the technique. The confocal microscope’s ability to block out-of-focus light and thereby perform optical sectioning through a specimen allows the researcher to quantify fluorescence with very high spatial precision. Serial.When used appropriately, a confocal fluorescence microscope is an excellent tool for making quantitative measurements in cells and tissues. Serial.print(val, HEX) // print the ASCII encoded hexadecimal analogValue Serial.print(val, DEC) // print the ASCII encoded decimal analogValue Serial.print(val, BIN) // print the ASCII encoded binary analogValue Serial.print(val, BYTE) // Print the raw binary value analogValue read the potentiometer's value and store it Serial.begin(9600) // initialize the serial port PinMode(ledPin, OUTPUT) // declare the ledPin as an output Int val = 0 // variable to store the potentiometer's reading Int ledStatus = LOW // we use a variable to toggle the LEDs state Int potPin = 4 // analog pin for the potentiometer * that the data coming from the ADC will be divided by 4. * we will only send a byte back to the computer, what means * In order to make the data transfer as simple as possible * input and sends the data as a byte back to the computer. * The code reads a potentiometer plugged to an analog * over the serial port (works in PC: Windows and Linux, and MAC) * This program sends data from a potentiometer to Processing (the next question would be, how to send different values at the same time for more than one parameter?) lars On the arduino-side i used some 'Potentiometer to Processing' code-example which sends a raw binary that can be read in python and with the 'print x, type (x)' commands i can see the binary as an ascii type (that changes by moving the potentiometer) and as type i got 'str' in the console: * Īt this point the 'str' datatype needs to be converted into a 'float' for controlling parameters in blender, which i couldn't solve with my very basic python knowledge tonight.Īny help with python would be appreciated here. So far, i made a simple blender-setup where the 'Cube' with an 'Always'-sensor triggers the python-controller.Įvery cycle of the game-engine calls the script once. if the input is '-1' then there is no data Serial.begin(9600) // connect to the serial port PinMode(ledPin9,OUTPUT) // declare the LED's pin as output PinMode(ledPin8,OUTPUT) // declare the LED's pin as output PinMode(ledPin13,OUTPUT) // declare the LED's pin as output Int ledPin8 = 8 // select the pin for the LED Int ledPin9 = 9 // select the pin for the LED Int ledPin10 = 10 // select the pin for the LED Int ledPin11 = 11 // select the pin for the LED Int ledPin12 = 12 // select the pin for the LED Int ledPin13 = 13 // select the pin for the LED Here is a simple example to control it with python (very simple one) It should be easy, since blender is written in python and you can easily control the arduino with python.
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